This experiment was done to test the hypothesis: Boiling and decrease in light will have negative affects on the rate of photosynthesis in chloroplasts. In this experiment we were told that we would be measuring the rate of photosynthesis. The reduction of a dye called DPIP is a part of the measurement technique. The transfer of electrons during the light-dependent reactions of photosynthesis reduce DPIP, changing it from light blue to colorless.The information on the lab sheet said that the light is a part of a continuum of radiation or energy. Wavelengths of energy have greater amounts of energy. Wavelengths of light with in the visible part of the light spectrum power photosynthesis. Electrons within each photosystem are excited to a higher energy level and this energy is used to produce ATP and reduce NADP to NADPH, when light is absorbed by the leaf pigments. Then ATP and NADPH are used to incorporate CO2 into organic molecules. This process is known as carbon fixation.In this experiment we were to use a dye reduction technique to study photosynthesis. We used this method to see if light reactions were taking place. The DPIP is taking place of the electron acceptor NADP. When light strikes the chloroplasts the electrons will be excited to high energy levels and will reduce DPIP. The color will change from light blue to colorless. While this occurs there is an increase in light transmittance through the solution. This increase in transmittance of light is measured by using a spectrophotometer.Materials and Method:The teacher provided us with an outlined procedure of the lab. The teacher started the spectrophotometer and informed us on what it does and how it operates. Chloroplasts were prepared while the spectrophotometer got ready. There was an ice bin that contained the boiled and unboiled chloroplasts at all times, except when they were placed in cuvettes. They are of incubation, a light shining through a fish...
